Intonation refers to the ability of a
guitar to keep it's tuning as it is played down the
fretboard. For example, if playing the open E
string yields a perfect E, playing an E at the 12th
fret should also yield a perfect E. This is determined by the distance between the
nut and the
bridge of the guitar, as well as the condition of the strings and
frets. Most electric guitars allow intonation to be adjusted, either by moving the whole
bridge, or each individual string such as with a
Gibson tune-o-matic bridge.
Acoustic guitars, in general, cannot adjust intonation without altering the bridge in a somewhat permanent manner.